Journal: Frontiers in Immunology
Article Title: Modulation of IRF7-driven transcription as a strategy to control HIV-1 latency
doi: 10.3389/fimmu.2026.1735192
Figure Lengend Snippet: Pacritinib blocks HIV latency reversal in an IRF7-dependent manner. (A) Left panel, WB of a representative experiment of HL60 cells treated with PMA or with a dose response of pacritinib (Pac) immunoblotted with anti-pJAK2, anti-pSTAT1, anti-IRF7 and anti-GAPDH antibodies. Middle panel, bar plots represent the mean quantification of IRF7 bands obtained by densitometry analysis of three independent experiments. Values were normalized to that of GAPDH used as a loading control and relativized to the untreated condition (ND). Right panel, relative mRNA expression of IRF7 gene expression measured by quantitative RT-PCR and normalized to GAPDH. (B, C) WB of a representative experiment in J-HIG (B) and HL-HIG (C) cells treated with PMA, pacritinib or fedratinib (Fed), alone or in combination, and immunoblotted with anti-IRF7 and anti-GAPDH antibodies. Bar plots represent the mean quantification of bands obtained by densitometry analysis of 3 independent experiments. (D, E) Correlation plots of IRF7 and gene expression versus HIV-1 latency reversal capacity of PMA or JAK2i-treated J-HIG and HL-HIG cells. (F) Transactivation assay as measured by luciferase expression in TZM-bl cells transfected to overexpress IRF7 protein and 24 hours after drug treatment. Luciferase values were normalized within each transfection condition (mock or IRF7 overexpression, left panel), and overexpression of IRF7 confirmed by WB ( right panel). PMA: phorbol 12-myristate 13-acetate (0,1µg/mL); Pac: pacritinib (1µM); Fed: fedratinib (5µM); Vor: vorinostat (5µM). Data expressed as means ± SD values from at least three independent experiments normalized to the no drug (ND) condition *p<0.05; **p<0.005; ***p<0.0001.
Article Snippet: The human cell lines HL60, Jurkat, MOLT-4 and U937 were obtained from ATCC (Gaithersburg, MD).
Techniques: Control, Expressing, Gene Expression, Quantitative RT-PCR, Transactivation Assay, Luciferase, Transfection, Over Expression
ATCC is a verified supplier 
ATCC manufactures this product 
