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human tumor cell lines hl 60  (ATCC)


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    ATCC human tumor cell lines hl 60
    Human Tumor Cell Lines Hl 60, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6534 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+human+hl+60+hl+60/HL-60/pm42034196-89-0-13
    Average 99 stars, based on 6534 article reviews
    human tumor cell lines hl 60 - by Bioz Stars, 2026-10
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    Enzyme-linked Immunosorbent Assay:

    Article Title: Histones, DNA, and Citrullination Promote Neutrophil Extracellular Trap Inflammation by Regulating the Localization and Activation of TLR4.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA tag antibody Abcam Ab9110; RRID: AB_307019 Histone H3 (citrulline R2+R8+R17) (anti-cit-H3) Abcam Ab5103; RRID: AB_304752 Rabbit IgG, polyclonal - Isotype Control Abcam Ab37415; RRID: AB_2631996 TLR4 antibody Abcam Ab22048; RRID: AB_446735 Mouse IgG1 kappa isotype control antibody Antibodies online ABIN2704378; RRID: AB_2833099 S100A8 antibody Antibodies online ABIN111892; RRID: AB_2833100 PE Mouse Anti-Human TLR4 (CD284), Clone TF901 BD biosciences 564215; RRID: AB_2738674 Alexa Fluor 647-anti-mouse-Ly6G-antibody Biolegend 127610; RRID: AB_1134159 Maltose Binding Protein (MBP) antibody Biolegend 906901; RRID: AB_2565070 Rab5 antibody Cell Signaling Technology C8B1 - 3547; RRID: AB_2300649 Neutrophil elastase antibody GeneTex GTX72042; RRID: AB_383332 TLR2 neutralizing antibody Invivogen pab-hstlr2; RRID: AB_11124921 TLR4 neutralizing antibody Invivogen pab-hstlr4; RRID: AB_11125132 PL2-3 anti-chromatin mouse antibody Losman et al., 1992 N/A Histone H3 antibody Millipore 07-690; RRID: AB_417398 Myeloperoxidase antibody R&D AF3667; RRID: AB_2250866 Chemicals, Peptides, and Recombinant Proteins AnykD Criterion TGX Precast Midi Protein Gel Bio-Rad Laboratories 5671124 Trans-Blot Turbo Midi PVDF membrane Bio-Rad Laboratories 1704157 Histone H3 (human recombinant) Cayman Chemical 10263 SYTOX green Invitrogen S7020 LPS-SM (LPS from S. minnesota R595) Invivogen tlrl-smlps FSL-1 Invivogen tlrl-fsl CD14 microbeads MACS Miltenyi 130-050-201 Cloride-amidine Merk Millipore 506282 BseRI New England biolabs R0581S PacI New England biolabs R0547S NdeI New England biolabs R0111S AflII New England biolabs R0541S Amylose Resin high flow New England Biolabs E8022S Micrococcal Nuclease (MNase) New England Biolabs M0247S Cholesterol Sigma C8667 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich 4693159001 Bafilomycin A1 from Streptomyces griseus Sigma-Aldrich B1793 Benzonase Nuclease Sigma-Aldrich E1014 ProLong Gold Antifade Mountant ThermoFisher scientific P36930 DAPI(4’,6-Diamidino-2-Phenylindole, Dihydrochloride) ThermoFisher scientific D1306 LIVE/DEAD Fixable Blue Dead Cell Stain Kit ThermoFisher scientific L34961 Critical Commercial Assays Human IFN-beta ELISA Kit R&D systems 41410-1 Limulus amebocyte lysate assay ThermoFisher scientific 88282 Pierce Fab Preparation Kit ThermoFisher scientific 44985 (Continued on next page) e1 Cell Reports 31, 107602, May 5, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER IL-1 beta Human Uncoated ELISA Kit ThermoFisher scientific 88-7261-77 IL-1 beta Mouse Uncoated ELISA Kit ThermoFisher scientific 88-7013-88 Interferon alpha (Cell culture) Human ELISA Kit ThermoFisher scientific 411001 IFN-alpha/IFN-beta 2-Plex Mouse ProcartaPlex Panel ThermoFisher scientific EPX02A-22187-901 Experimental Models: Cell Lines Human HL-60 HL-60 (ATCC CCL-240) ATCC CCL-240 HEK-Blue hTLR2 Invivogen hkb-htlr2 HEK-Blue hTLR4 Invivogen hkb-htlr4 HEK-Blue hTLR9 Invivogen hkb-htlr9 HEK-Blue Null1 Cells Invivogen hkb-null1 HEK-Blue Null2 Cells Invivogen hkb-null2 293/hTLR2-HA Invivogen 293-htlr2ha 293/hTLR4-HA Invivogen 293-htlr4ha 293/Null Invivogen 293-null Experimental Models: Organisms/Strains WT C. albicans SC5314 Arturo Zychlinsky N/A TLR9 / (C57BL/6J-Tlr9M7Btlr/Mmjax) Hemmi et al., 2000 N/A TLR4 / (B6(Cg)-Tlr4tm1.2Karp/J) Hoshino et al., 1999 N/A STING / (B6(Cg)-Sting1tm1.2Camb/J) Ishikawa and Barber, 2008 N/A ApoE / (B6.129P2-Apoetm1Unc/J) Piedrahita et al., 1992 N/A ApoE/PAD4 / This paper N/A PAD4 / (B6.Cg-Padi4tm1.1Kmow/J) Hemmers et al., 2011 N/A CAG::H2-EGFP (B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J) The Jackson Laboratory 006069; RRID: IMSR_JAX:006069 Oligonucleotides human ODN 2006 (ODN 7909) Invivogen tlrl-2006 BamHI-H3.1-Forward primer: 5-CGCGGATCCATGGCTC GTACTAAGCAG-3 Sigma-Aldrich N/A BamHI-hPAD4-forward: 5-CACGGATCCATGGCCCAG GGGACATTG-3 Sigma-Aldrich N/A SalI-hPAD4-Reverse: 5-GCGGTCGACTCAGGGCACC ATGTTCC-3 Sigma-Aldrich N/A XhoI-H3.1-Reverse primer: 5-GAGCTCGAGTTACGCCC TCTCCCCGC-3 Sigma-Aldrich N/A TaqMan Gene Expression Assay, Assay ID: Hs00174092_m1, human IL1A ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, Assay ID: Hs00174131_m1, human IL6 ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, Human HPRT1 Hs02800695_m1 ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, human IL1B Hs01555410_m1 ThermoFisher scientific 4331182 Recombinant DNA pMAL-c2X plasmid Addgene 75286 Software and Algorithms Fiji/ImageJ version 2.0.0 https://imagej.net/Contributors https://imagej.net/Fiji FlowJo version 10 Becton Dickinson https://www.flowjo.com GraphPad Prism Version 7 GraphPad Inc. https://www.graphpad.com/ scientific-software/prism/ Other L929 cell culture supernatant The Francis Crick Institute – Cell services core facility N/A Cell Reports 31, 107602, May 5, 2020 e2 ..

    Cell Culture:

    Article Title: Histones, DNA, and Citrullination Promote Neutrophil Extracellular Trap Inflammation by Regulating the Localization and Activation of TLR4.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA tag antibody Abcam Ab9110; RRID: AB_307019 Histone H3 (citrulline R2+R8+R17) (anti-cit-H3) Abcam Ab5103; RRID: AB_304752 Rabbit IgG, polyclonal - Isotype Control Abcam Ab37415; RRID: AB_2631996 TLR4 antibody Abcam Ab22048; RRID: AB_446735 Mouse IgG1 kappa isotype control antibody Antibodies online ABIN2704378; RRID: AB_2833099 S100A8 antibody Antibodies online ABIN111892; RRID: AB_2833100 PE Mouse Anti-Human TLR4 (CD284), Clone TF901 BD biosciences 564215; RRID: AB_2738674 Alexa Fluor 647-anti-mouse-Ly6G-antibody Biolegend 127610; RRID: AB_1134159 Maltose Binding Protein (MBP) antibody Biolegend 906901; RRID: AB_2565070 Rab5 antibody Cell Signaling Technology C8B1 - 3547; RRID: AB_2300649 Neutrophil elastase antibody GeneTex GTX72042; RRID: AB_383332 TLR2 neutralizing antibody Invivogen pab-hstlr2; RRID: AB_11124921 TLR4 neutralizing antibody Invivogen pab-hstlr4; RRID: AB_11125132 PL2-3 anti-chromatin mouse antibody Losman et al., 1992 N/A Histone H3 antibody Millipore 07-690; RRID: AB_417398 Myeloperoxidase antibody R&D AF3667; RRID: AB_2250866 Chemicals, Peptides, and Recombinant Proteins AnykD Criterion TGX Precast Midi Protein Gel Bio-Rad Laboratories 5671124 Trans-Blot Turbo Midi PVDF membrane Bio-Rad Laboratories 1704157 Histone H3 (human recombinant) Cayman Chemical 10263 SYTOX green Invitrogen S7020 LPS-SM (LPS from S. minnesota R595) Invivogen tlrl-smlps FSL-1 Invivogen tlrl-fsl CD14 microbeads MACS Miltenyi 130-050-201 Cloride-amidine Merk Millipore 506282 BseRI New England biolabs R0581S PacI New England biolabs R0547S NdeI New England biolabs R0111S AflII New England biolabs R0541S Amylose Resin high flow New England Biolabs E8022S Micrococcal Nuclease (MNase) New England Biolabs M0247S Cholesterol Sigma C8667 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich 4693159001 Bafilomycin A1 from Streptomyces griseus Sigma-Aldrich B1793 Benzonase Nuclease Sigma-Aldrich E1014 ProLong Gold Antifade Mountant ThermoFisher scientific P36930 DAPI(4’,6-Diamidino-2-Phenylindole, Dihydrochloride) ThermoFisher scientific D1306 LIVE/DEAD Fixable Blue Dead Cell Stain Kit ThermoFisher scientific L34961 Critical Commercial Assays Human IFN-beta ELISA Kit R&D systems 41410-1 Limulus amebocyte lysate assay ThermoFisher scientific 88282 Pierce Fab Preparation Kit ThermoFisher scientific 44985 (Continued on next page) e1 Cell Reports 31, 107602, May 5, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER IL-1 beta Human Uncoated ELISA Kit ThermoFisher scientific 88-7261-77 IL-1 beta Mouse Uncoated ELISA Kit ThermoFisher scientific 88-7013-88 Interferon alpha (Cell culture) Human ELISA Kit ThermoFisher scientific 411001 IFN-alpha/IFN-beta 2-Plex Mouse ProcartaPlex Panel ThermoFisher scientific EPX02A-22187-901 Experimental Models: Cell Lines Human HL-60 HL-60 (ATCC CCL-240) ATCC CCL-240 HEK-Blue hTLR2 Invivogen hkb-htlr2 HEK-Blue hTLR4 Invivogen hkb-htlr4 HEK-Blue hTLR9 Invivogen hkb-htlr9 HEK-Blue Null1 Cells Invivogen hkb-null1 HEK-Blue Null2 Cells Invivogen hkb-null2 293/hTLR2-HA Invivogen 293-htlr2ha 293/hTLR4-HA Invivogen 293-htlr4ha 293/Null Invivogen 293-null Experimental Models: Organisms/Strains WT C. albicans SC5314 Arturo Zychlinsky N/A TLR9 / (C57BL/6J-Tlr9M7Btlr/Mmjax) Hemmi et al., 2000 N/A TLR4 / (B6(Cg)-Tlr4tm1.2Karp/J) Hoshino et al., 1999 N/A STING / (B6(Cg)-Sting1tm1.2Camb/J) Ishikawa and Barber, 2008 N/A ApoE / (B6.129P2-Apoetm1Unc/J) Piedrahita et al., 1992 N/A ApoE/PAD4 / This paper N/A PAD4 / (B6.Cg-Padi4tm1.1Kmow/J) Hemmers et al., 2011 N/A CAG::H2-EGFP (B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J) The Jackson Laboratory 006069; RRID: IMSR_JAX:006069 Oligonucleotides human ODN 2006 (ODN 7909) Invivogen tlrl-2006 BamHI-H3.1-Forward primer: 5-CGCGGATCCATGGCTC GTACTAAGCAG-3 Sigma-Aldrich N/A BamHI-hPAD4-forward: 5-CACGGATCCATGGCCCAG GGGACATTG-3 Sigma-Aldrich N/A SalI-hPAD4-Reverse: 5-GCGGTCGACTCAGGGCACC ATGTTCC-3 Sigma-Aldrich N/A XhoI-H3.1-Reverse primer: 5-GAGCTCGAGTTACGCCC TCTCCCCGC-3 Sigma-Aldrich N/A TaqMan Gene Expression Assay, Assay ID: Hs00174092_m1, human IL1A ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, Assay ID: Hs00174131_m1, human IL6 ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, Human HPRT1 Hs02800695_m1 ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, human IL1B Hs01555410_m1 ThermoFisher scientific 4331182 Recombinant DNA pMAL-c2X plasmid Addgene 75286 Software and Algorithms Fiji/ImageJ version 2.0.0 https://imagej.net/Contributors https://imagej.net/Fiji FlowJo version 10 Becton Dickinson https://www.flowjo.com GraphPad Prism Version 7 GraphPad Inc. https://www.graphpad.com/ scientific-software/prism/ Other L929 cell culture supernatant The Francis Crick Institute – Cell services core facility N/A Cell Reports 31, 107602, May 5, 2020 e2 ..

    Gene Expression:

    Article Title: Histones, DNA, and Citrullination Promote Neutrophil Extracellular Trap Inflammation by Regulating the Localization and Activation of TLR4.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA tag antibody Abcam Ab9110; RRID: AB_307019 Histone H3 (citrulline R2+R8+R17) (anti-cit-H3) Abcam Ab5103; RRID: AB_304752 Rabbit IgG, polyclonal - Isotype Control Abcam Ab37415; RRID: AB_2631996 TLR4 antibody Abcam Ab22048; RRID: AB_446735 Mouse IgG1 kappa isotype control antibody Antibodies online ABIN2704378; RRID: AB_2833099 S100A8 antibody Antibodies online ABIN111892; RRID: AB_2833100 PE Mouse Anti-Human TLR4 (CD284), Clone TF901 BD biosciences 564215; RRID: AB_2738674 Alexa Fluor 647-anti-mouse-Ly6G-antibody Biolegend 127610; RRID: AB_1134159 Maltose Binding Protein (MBP) antibody Biolegend 906901; RRID: AB_2565070 Rab5 antibody Cell Signaling Technology C8B1 - 3547; RRID: AB_2300649 Neutrophil elastase antibody GeneTex GTX72042; RRID: AB_383332 TLR2 neutralizing antibody Invivogen pab-hstlr2; RRID: AB_11124921 TLR4 neutralizing antibody Invivogen pab-hstlr4; RRID: AB_11125132 PL2-3 anti-chromatin mouse antibody Losman et al., 1992 N/A Histone H3 antibody Millipore 07-690; RRID: AB_417398 Myeloperoxidase antibody R&D AF3667; RRID: AB_2250866 Chemicals, Peptides, and Recombinant Proteins AnykD Criterion TGX Precast Midi Protein Gel Bio-Rad Laboratories 5671124 Trans-Blot Turbo Midi PVDF membrane Bio-Rad Laboratories 1704157 Histone H3 (human recombinant) Cayman Chemical 10263 SYTOX green Invitrogen S7020 LPS-SM (LPS from S. minnesota R595) Invivogen tlrl-smlps FSL-1 Invivogen tlrl-fsl CD14 microbeads MACS Miltenyi 130-050-201 Cloride-amidine Merk Millipore 506282 BseRI New England biolabs R0581S PacI New England biolabs R0547S NdeI New England biolabs R0111S AflII New England biolabs R0541S Amylose Resin high flow New England Biolabs E8022S Micrococcal Nuclease (MNase) New England Biolabs M0247S Cholesterol Sigma C8667 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich 4693159001 Bafilomycin A1 from Streptomyces griseus Sigma-Aldrich B1793 Benzonase Nuclease Sigma-Aldrich E1014 ProLong Gold Antifade Mountant ThermoFisher scientific P36930 DAPI(4’,6-Diamidino-2-Phenylindole, Dihydrochloride) ThermoFisher scientific D1306 LIVE/DEAD Fixable Blue Dead Cell Stain Kit ThermoFisher scientific L34961 Critical Commercial Assays Human IFN-beta ELISA Kit R&D systems 41410-1 Limulus amebocyte lysate assay ThermoFisher scientific 88282 Pierce Fab Preparation Kit ThermoFisher scientific 44985 (Continued on next page) e1 Cell Reports 31, 107602, May 5, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER IL-1 beta Human Uncoated ELISA Kit ThermoFisher scientific 88-7261-77 IL-1 beta Mouse Uncoated ELISA Kit ThermoFisher scientific 88-7013-88 Interferon alpha (Cell culture) Human ELISA Kit ThermoFisher scientific 411001 IFN-alpha/IFN-beta 2-Plex Mouse ProcartaPlex Panel ThermoFisher scientific EPX02A-22187-901 Experimental Models: Cell Lines Human HL-60 HL-60 (ATCC CCL-240) ATCC CCL-240 HEK-Blue hTLR2 Invivogen hkb-htlr2 HEK-Blue hTLR4 Invivogen hkb-htlr4 HEK-Blue hTLR9 Invivogen hkb-htlr9 HEK-Blue Null1 Cells Invivogen hkb-null1 HEK-Blue Null2 Cells Invivogen hkb-null2 293/hTLR2-HA Invivogen 293-htlr2ha 293/hTLR4-HA Invivogen 293-htlr4ha 293/Null Invivogen 293-null Experimental Models: Organisms/Strains WT C. albicans SC5314 Arturo Zychlinsky N/A TLR9 / (C57BL/6J-Tlr9M7Btlr/Mmjax) Hemmi et al., 2000 N/A TLR4 / (B6(Cg)-Tlr4tm1.2Karp/J) Hoshino et al., 1999 N/A STING / (B6(Cg)-Sting1tm1.2Camb/J) Ishikawa and Barber, 2008 N/A ApoE / (B6.129P2-Apoetm1Unc/J) Piedrahita et al., 1992 N/A ApoE/PAD4 / This paper N/A PAD4 / (B6.Cg-Padi4tm1.1Kmow/J) Hemmers et al., 2011 N/A CAG::H2-EGFP (B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J) The Jackson Laboratory 006069; RRID: IMSR_JAX:006069 Oligonucleotides human ODN 2006 (ODN 7909) Invivogen tlrl-2006 BamHI-H3.1-Forward primer: 5-CGCGGATCCATGGCTC GTACTAAGCAG-3 Sigma-Aldrich N/A BamHI-hPAD4-forward: 5-CACGGATCCATGGCCCAG GGGACATTG-3 Sigma-Aldrich N/A SalI-hPAD4-Reverse: 5-GCGGTCGACTCAGGGCACC ATGTTCC-3 Sigma-Aldrich N/A XhoI-H3.1-Reverse primer: 5-GAGCTCGAGTTACGCCC TCTCCCCGC-3 Sigma-Aldrich N/A TaqMan Gene Expression Assay, Assay ID: Hs00174092_m1, human IL1A ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, Assay ID: Hs00174131_m1, human IL6 ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, Human HPRT1 Hs02800695_m1 ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, human IL1B Hs01555410_m1 ThermoFisher scientific 4331182 Recombinant DNA pMAL-c2X plasmid Addgene 75286 Software and Algorithms Fiji/ImageJ version 2.0.0 https://imagej.net/Contributors https://imagej.net/Fiji FlowJo version 10 Becton Dickinson https://www.flowjo.com GraphPad Prism Version 7 GraphPad Inc. https://www.graphpad.com/ scientific-software/prism/ Other L929 cell culture supernatant The Francis Crick Institute – Cell services core facility N/A Cell Reports 31, 107602, May 5, 2020 e2 ..

    Recombinant:

    Article Title: Histones, DNA, and Citrullination Promote Neutrophil Extracellular Trap Inflammation by Regulating the Localization and Activation of TLR4.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA tag antibody Abcam Ab9110; RRID: AB_307019 Histone H3 (citrulline R2+R8+R17) (anti-cit-H3) Abcam Ab5103; RRID: AB_304752 Rabbit IgG, polyclonal - Isotype Control Abcam Ab37415; RRID: AB_2631996 TLR4 antibody Abcam Ab22048; RRID: AB_446735 Mouse IgG1 kappa isotype control antibody Antibodies online ABIN2704378; RRID: AB_2833099 S100A8 antibody Antibodies online ABIN111892; RRID: AB_2833100 PE Mouse Anti-Human TLR4 (CD284), Clone TF901 BD biosciences 564215; RRID: AB_2738674 Alexa Fluor 647-anti-mouse-Ly6G-antibody Biolegend 127610; RRID: AB_1134159 Maltose Binding Protein (MBP) antibody Biolegend 906901; RRID: AB_2565070 Rab5 antibody Cell Signaling Technology C8B1 - 3547; RRID: AB_2300649 Neutrophil elastase antibody GeneTex GTX72042; RRID: AB_383332 TLR2 neutralizing antibody Invivogen pab-hstlr2; RRID: AB_11124921 TLR4 neutralizing antibody Invivogen pab-hstlr4; RRID: AB_11125132 PL2-3 anti-chromatin mouse antibody Losman et al., 1992 N/A Histone H3 antibody Millipore 07-690; RRID: AB_417398 Myeloperoxidase antibody R&D AF3667; RRID: AB_2250866 Chemicals, Peptides, and Recombinant Proteins AnykD Criterion TGX Precast Midi Protein Gel Bio-Rad Laboratories 5671124 Trans-Blot Turbo Midi PVDF membrane Bio-Rad Laboratories 1704157 Histone H3 (human recombinant) Cayman Chemical 10263 SYTOX green Invitrogen S7020 LPS-SM (LPS from S. minnesota R595) Invivogen tlrl-smlps FSL-1 Invivogen tlrl-fsl CD14 microbeads MACS Miltenyi 130-050-201 Cloride-amidine Merk Millipore 506282 BseRI New England biolabs R0581S PacI New England biolabs R0547S NdeI New England biolabs R0111S AflII New England biolabs R0541S Amylose Resin high flow New England Biolabs E8022S Micrococcal Nuclease (MNase) New England Biolabs M0247S Cholesterol Sigma C8667 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich 4693159001 Bafilomycin A1 from Streptomyces griseus Sigma-Aldrich B1793 Benzonase Nuclease Sigma-Aldrich E1014 ProLong Gold Antifade Mountant ThermoFisher scientific P36930 DAPI(4’,6-Diamidino-2-Phenylindole, Dihydrochloride) ThermoFisher scientific D1306 LIVE/DEAD Fixable Blue Dead Cell Stain Kit ThermoFisher scientific L34961 Critical Commercial Assays Human IFN-beta ELISA Kit R&D systems 41410-1 Limulus amebocyte lysate assay ThermoFisher scientific 88282 Pierce Fab Preparation Kit ThermoFisher scientific 44985 (Continued on next page) e1 Cell Reports 31, 107602, May 5, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER IL-1 beta Human Uncoated ELISA Kit ThermoFisher scientific 88-7261-77 IL-1 beta Mouse Uncoated ELISA Kit ThermoFisher scientific 88-7013-88 Interferon alpha (Cell culture) Human ELISA Kit ThermoFisher scientific 411001 IFN-alpha/IFN-beta 2-Plex Mouse ProcartaPlex Panel ThermoFisher scientific EPX02A-22187-901 Experimental Models: Cell Lines Human HL-60 HL-60 (ATCC CCL-240) ATCC CCL-240 HEK-Blue hTLR2 Invivogen hkb-htlr2 HEK-Blue hTLR4 Invivogen hkb-htlr4 HEK-Blue hTLR9 Invivogen hkb-htlr9 HEK-Blue Null1 Cells Invivogen hkb-null1 HEK-Blue Null2 Cells Invivogen hkb-null2 293/hTLR2-HA Invivogen 293-htlr2ha 293/hTLR4-HA Invivogen 293-htlr4ha 293/Null Invivogen 293-null Experimental Models: Organisms/Strains WT C. albicans SC5314 Arturo Zychlinsky N/A TLR9 / (C57BL/6J-Tlr9M7Btlr/Mmjax) Hemmi et al., 2000 N/A TLR4 / (B6(Cg)-Tlr4tm1.2Karp/J) Hoshino et al., 1999 N/A STING / (B6(Cg)-Sting1tm1.2Camb/J) Ishikawa and Barber, 2008 N/A ApoE / (B6.129P2-Apoetm1Unc/J) Piedrahita et al., 1992 N/A ApoE/PAD4 / This paper N/A PAD4 / (B6.Cg-Padi4tm1.1Kmow/J) Hemmers et al., 2011 N/A CAG::H2-EGFP (B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J) The Jackson Laboratory 006069; RRID: IMSR_JAX:006069 Oligonucleotides human ODN 2006 (ODN 7909) Invivogen tlrl-2006 BamHI-H3.1-Forward primer: 5-CGCGGATCCATGGCTC GTACTAAGCAG-3 Sigma-Aldrich N/A BamHI-hPAD4-forward: 5-CACGGATCCATGGCCCAG GGGACATTG-3 Sigma-Aldrich N/A SalI-hPAD4-Reverse: 5-GCGGTCGACTCAGGGCACC ATGTTCC-3 Sigma-Aldrich N/A XhoI-H3.1-Reverse primer: 5-GAGCTCGAGTTACGCCC TCTCCCCGC-3 Sigma-Aldrich N/A TaqMan Gene Expression Assay, Assay ID: Hs00174092_m1, human IL1A ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, Assay ID: Hs00174131_m1, human IL6 ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, Human HPRT1 Hs02800695_m1 ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, human IL1B Hs01555410_m1 ThermoFisher scientific 4331182 Recombinant DNA pMAL-c2X plasmid Addgene 75286 Software and Algorithms Fiji/ImageJ version 2.0.0 https://imagej.net/Contributors https://imagej.net/Fiji FlowJo version 10 Becton Dickinson https://www.flowjo.com GraphPad Prism Version 7 GraphPad Inc. https://www.graphpad.com/ scientific-software/prism/ Other L929 cell culture supernatant The Francis Crick Institute – Cell services core facility N/A Cell Reports 31, 107602, May 5, 2020 e2 ..

    Plasmid Preparation:

    Article Title: Histones, DNA, and Citrullination Promote Neutrophil Extracellular Trap Inflammation by Regulating the Localization and Activation of TLR4.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA tag antibody Abcam Ab9110; RRID: AB_307019 Histone H3 (citrulline R2+R8+R17) (anti-cit-H3) Abcam Ab5103; RRID: AB_304752 Rabbit IgG, polyclonal - Isotype Control Abcam Ab37415; RRID: AB_2631996 TLR4 antibody Abcam Ab22048; RRID: AB_446735 Mouse IgG1 kappa isotype control antibody Antibodies online ABIN2704378; RRID: AB_2833099 S100A8 antibody Antibodies online ABIN111892; RRID: AB_2833100 PE Mouse Anti-Human TLR4 (CD284), Clone TF901 BD biosciences 564215; RRID: AB_2738674 Alexa Fluor 647-anti-mouse-Ly6G-antibody Biolegend 127610; RRID: AB_1134159 Maltose Binding Protein (MBP) antibody Biolegend 906901; RRID: AB_2565070 Rab5 antibody Cell Signaling Technology C8B1 - 3547; RRID: AB_2300649 Neutrophil elastase antibody GeneTex GTX72042; RRID: AB_383332 TLR2 neutralizing antibody Invivogen pab-hstlr2; RRID: AB_11124921 TLR4 neutralizing antibody Invivogen pab-hstlr4; RRID: AB_11125132 PL2-3 anti-chromatin mouse antibody Losman et al., 1992 N/A Histone H3 antibody Millipore 07-690; RRID: AB_417398 Myeloperoxidase antibody R&D AF3667; RRID: AB_2250866 Chemicals, Peptides, and Recombinant Proteins AnykD Criterion TGX Precast Midi Protein Gel Bio-Rad Laboratories 5671124 Trans-Blot Turbo Midi PVDF membrane Bio-Rad Laboratories 1704157 Histone H3 (human recombinant) Cayman Chemical 10263 SYTOX green Invitrogen S7020 LPS-SM (LPS from S. minnesota R595) Invivogen tlrl-smlps FSL-1 Invivogen tlrl-fsl CD14 microbeads MACS Miltenyi 130-050-201 Cloride-amidine Merk Millipore 506282 BseRI New England biolabs R0581S PacI New England biolabs R0547S NdeI New England biolabs R0111S AflII New England biolabs R0541S Amylose Resin high flow New England Biolabs E8022S Micrococcal Nuclease (MNase) New England Biolabs M0247S Cholesterol Sigma C8667 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich 4693159001 Bafilomycin A1 from Streptomyces griseus Sigma-Aldrich B1793 Benzonase Nuclease Sigma-Aldrich E1014 ProLong Gold Antifade Mountant ThermoFisher scientific P36930 DAPI(4’,6-Diamidino-2-Phenylindole, Dihydrochloride) ThermoFisher scientific D1306 LIVE/DEAD Fixable Blue Dead Cell Stain Kit ThermoFisher scientific L34961 Critical Commercial Assays Human IFN-beta ELISA Kit R&D systems 41410-1 Limulus amebocyte lysate assay ThermoFisher scientific 88282 Pierce Fab Preparation Kit ThermoFisher scientific 44985 (Continued on next page) e1 Cell Reports 31, 107602, May 5, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER IL-1 beta Human Uncoated ELISA Kit ThermoFisher scientific 88-7261-77 IL-1 beta Mouse Uncoated ELISA Kit ThermoFisher scientific 88-7013-88 Interferon alpha (Cell culture) Human ELISA Kit ThermoFisher scientific 411001 IFN-alpha/IFN-beta 2-Plex Mouse ProcartaPlex Panel ThermoFisher scientific EPX02A-22187-901 Experimental Models: Cell Lines Human HL-60 HL-60 (ATCC CCL-240) ATCC CCL-240 HEK-Blue hTLR2 Invivogen hkb-htlr2 HEK-Blue hTLR4 Invivogen hkb-htlr4 HEK-Blue hTLR9 Invivogen hkb-htlr9 HEK-Blue Null1 Cells Invivogen hkb-null1 HEK-Blue Null2 Cells Invivogen hkb-null2 293/hTLR2-HA Invivogen 293-htlr2ha 293/hTLR4-HA Invivogen 293-htlr4ha 293/Null Invivogen 293-null Experimental Models: Organisms/Strains WT C. albicans SC5314 Arturo Zychlinsky N/A TLR9 / (C57BL/6J-Tlr9M7Btlr/Mmjax) Hemmi et al., 2000 N/A TLR4 / (B6(Cg)-Tlr4tm1.2Karp/J) Hoshino et al., 1999 N/A STING / (B6(Cg)-Sting1tm1.2Camb/J) Ishikawa and Barber, 2008 N/A ApoE / (B6.129P2-Apoetm1Unc/J) Piedrahita et al., 1992 N/A ApoE/PAD4 / This paper N/A PAD4 / (B6.Cg-Padi4tm1.1Kmow/J) Hemmers et al., 2011 N/A CAG::H2-EGFP (B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J) The Jackson Laboratory 006069; RRID: IMSR_JAX:006069 Oligonucleotides human ODN 2006 (ODN 7909) Invivogen tlrl-2006 BamHI-H3.1-Forward primer: 5-CGCGGATCCATGGCTC GTACTAAGCAG-3 Sigma-Aldrich N/A BamHI-hPAD4-forward: 5-CACGGATCCATGGCCCAG GGGACATTG-3 Sigma-Aldrich N/A SalI-hPAD4-Reverse: 5-GCGGTCGACTCAGGGCACC ATGTTCC-3 Sigma-Aldrich N/A XhoI-H3.1-Reverse primer: 5-GAGCTCGAGTTACGCCC TCTCCCCGC-3 Sigma-Aldrich N/A TaqMan Gene Expression Assay, Assay ID: Hs00174092_m1, human IL1A ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, Assay ID: Hs00174131_m1, human IL6 ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, Human HPRT1 Hs02800695_m1 ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, human IL1B Hs01555410_m1 ThermoFisher scientific 4331182 Recombinant DNA pMAL-c2X plasmid Addgene 75286 Software and Algorithms Fiji/ImageJ version 2.0.0 https://imagej.net/Contributors https://imagej.net/Fiji FlowJo version 10 Becton Dickinson https://www.flowjo.com GraphPad Prism Version 7 GraphPad Inc. https://www.graphpad.com/ scientific-software/prism/ Other L929 cell culture supernatant The Francis Crick Institute – Cell services core facility N/A Cell Reports 31, 107602, May 5, 2020 e2 ..

    Software:

    Article Title: Histones, DNA, and Citrullination Promote Neutrophil Extracellular Trap Inflammation by Regulating the Localization and Activation of TLR4.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-HA tag antibody Abcam Ab9110; RRID: AB_307019 Histone H3 (citrulline R2+R8+R17) (anti-cit-H3) Abcam Ab5103; RRID: AB_304752 Rabbit IgG, polyclonal - Isotype Control Abcam Ab37415; RRID: AB_2631996 TLR4 antibody Abcam Ab22048; RRID: AB_446735 Mouse IgG1 kappa isotype control antibody Antibodies online ABIN2704378; RRID: AB_2833099 S100A8 antibody Antibodies online ABIN111892; RRID: AB_2833100 PE Mouse Anti-Human TLR4 (CD284), Clone TF901 BD biosciences 564215; RRID: AB_2738674 Alexa Fluor 647-anti-mouse-Ly6G-antibody Biolegend 127610; RRID: AB_1134159 Maltose Binding Protein (MBP) antibody Biolegend 906901; RRID: AB_2565070 Rab5 antibody Cell Signaling Technology C8B1 - 3547; RRID: AB_2300649 Neutrophil elastase antibody GeneTex GTX72042; RRID: AB_383332 TLR2 neutralizing antibody Invivogen pab-hstlr2; RRID: AB_11124921 TLR4 neutralizing antibody Invivogen pab-hstlr4; RRID: AB_11125132 PL2-3 anti-chromatin mouse antibody Losman et al., 1992 N/A Histone H3 antibody Millipore 07-690; RRID: AB_417398 Myeloperoxidase antibody R&D AF3667; RRID: AB_2250866 Chemicals, Peptides, and Recombinant Proteins AnykD Criterion TGX Precast Midi Protein Gel Bio-Rad Laboratories 5671124 Trans-Blot Turbo Midi PVDF membrane Bio-Rad Laboratories 1704157 Histone H3 (human recombinant) Cayman Chemical 10263 SYTOX green Invitrogen S7020 LPS-SM (LPS from S. minnesota R595) Invivogen tlrl-smlps FSL-1 Invivogen tlrl-fsl CD14 microbeads MACS Miltenyi 130-050-201 Cloride-amidine Merk Millipore 506282 BseRI New England biolabs R0581S PacI New England biolabs R0547S NdeI New England biolabs R0111S AflII New England biolabs R0541S Amylose Resin high flow New England Biolabs E8022S Micrococcal Nuclease (MNase) New England Biolabs M0247S Cholesterol Sigma C8667 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Sigma-Aldrich 4693159001 Bafilomycin A1 from Streptomyces griseus Sigma-Aldrich B1793 Benzonase Nuclease Sigma-Aldrich E1014 ProLong Gold Antifade Mountant ThermoFisher scientific P36930 DAPI(4’,6-Diamidino-2-Phenylindole, Dihydrochloride) ThermoFisher scientific D1306 LIVE/DEAD Fixable Blue Dead Cell Stain Kit ThermoFisher scientific L34961 Critical Commercial Assays Human IFN-beta ELISA Kit R&D systems 41410-1 Limulus amebocyte lysate assay ThermoFisher scientific 88282 Pierce Fab Preparation Kit ThermoFisher scientific 44985 (Continued on next page) e1 Cell Reports 31, 107602, May 5, 2020 .. REAGENT or RESOURCE SOURCE IDENTIFIER IL-1 beta Human Uncoated ELISA Kit ThermoFisher scientific 88-7261-77 IL-1 beta Mouse Uncoated ELISA Kit ThermoFisher scientific 88-7013-88 Interferon alpha (Cell culture) Human ELISA Kit ThermoFisher scientific 411001 IFN-alpha/IFN-beta 2-Plex Mouse ProcartaPlex Panel ThermoFisher scientific EPX02A-22187-901 Experimental Models: Cell Lines Human HL-60 HL-60 (ATCC CCL-240) ATCC CCL-240 HEK-Blue hTLR2 Invivogen hkb-htlr2 HEK-Blue hTLR4 Invivogen hkb-htlr4 HEK-Blue hTLR9 Invivogen hkb-htlr9 HEK-Blue Null1 Cells Invivogen hkb-null1 HEK-Blue Null2 Cells Invivogen hkb-null2 293/hTLR2-HA Invivogen 293-htlr2ha 293/hTLR4-HA Invivogen 293-htlr4ha 293/Null Invivogen 293-null Experimental Models: Organisms/Strains WT C. albicans SC5314 Arturo Zychlinsky N/A TLR9 / (C57BL/6J-Tlr9M7Btlr/Mmjax) Hemmi et al., 2000 N/A TLR4 / (B6(Cg)-Tlr4tm1.2Karp/J) Hoshino et al., 1999 N/A STING / (B6(Cg)-Sting1tm1.2Camb/J) Ishikawa and Barber, 2008 N/A ApoE / (B6.129P2-Apoetm1Unc/J) Piedrahita et al., 1992 N/A ApoE/PAD4 / This paper N/A PAD4 / (B6.Cg-Padi4tm1.1Kmow/J) Hemmers et al., 2011 N/A CAG::H2-EGFP (B6.Cg-Tg(HIST1H2BB/EGFP)1Pa/J) The Jackson Laboratory 006069; RRID: IMSR_JAX:006069 Oligonucleotides human ODN 2006 (ODN 7909) Invivogen tlrl-2006 BamHI-H3.1-Forward primer: 5-CGCGGATCCATGGCTC GTACTAAGCAG-3 Sigma-Aldrich N/A BamHI-hPAD4-forward: 5-CACGGATCCATGGCCCAG GGGACATTG-3 Sigma-Aldrich N/A SalI-hPAD4-Reverse: 5-GCGGTCGACTCAGGGCACC ATGTTCC-3 Sigma-Aldrich N/A XhoI-H3.1-Reverse primer: 5-GAGCTCGAGTTACGCCC TCTCCCCGC-3 Sigma-Aldrich N/A TaqMan Gene Expression Assay, Assay ID: Hs00174092_m1, human IL1A ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, Assay ID: Hs00174131_m1, human IL6 ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, Human HPRT1 Hs02800695_m1 ThermoFisher scientific 4331182 TaqMan Gene Expression Assay, human IL1B Hs01555410_m1 ThermoFisher scientific 4331182 Recombinant DNA pMAL-c2X plasmid Addgene 75286 Software and Algorithms Fiji/ImageJ version 2.0.0 https://imagej.net/Contributors https://imagej.net/Fiji FlowJo version 10 Becton Dickinson https://www.flowjo.com GraphPad Prism Version 7 GraphPad Inc. https://www.graphpad.com/ scientific-software/prism/ Other L929 cell culture supernatant The Francis Crick Institute – Cell services core facility N/A Cell Reports 31, 107602, May 5, 2020 e2 ..



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    Pacritinib blocks HIV latency reversal in an IRF7-dependent manner. (A) Left panel, WB of a representative experiment of HL60 cells treated with PMA or with a dose response of pacritinib (Pac) immunoblotted with anti-pJAK2, anti-pSTAT1, anti-IRF7 and anti-GAPDH antibodies. Middle panel, bar plots represent the mean quantification of IRF7 bands obtained by densitometry analysis of three independent experiments. Values were normalized to that of GAPDH used as a loading control and relativized to the untreated condition (ND). Right panel, relative mRNA expression of IRF7 gene expression measured by quantitative RT-PCR and normalized to GAPDH. (B, C) WB of a representative experiment in J-HIG (B) and HL-HIG (C) cells treated with PMA, pacritinib or fedratinib (Fed), alone or in combination, and immunoblotted with anti-IRF7 and anti-GAPDH antibodies. Bar plots represent the mean quantification of bands obtained by densitometry analysis of 3 independent experiments. (D, E) Correlation plots of IRF7 and gene expression versus HIV-1 latency reversal capacity of PMA or JAK2i-treated J-HIG and HL-HIG cells. (F) Transactivation assay as measured by luciferase expression in TZM-bl cells transfected to overexpress IRF7 protein and 24 hours after drug treatment. Luciferase values were normalized within each transfection condition (mock or IRF7 overexpression, left panel), and overexpression of IRF7 confirmed by WB ( right panel). PMA: phorbol 12-myristate 13-acetate (0,1µg/mL); Pac: pacritinib (1µM); Fed: fedratinib (5µM); Vor: vorinostat (5µM). Data expressed as means ± SD values from at least three independent experiments normalized to the no drug (ND) condition *p<0.05; **p<0.005; ***p<0.0001.

    Journal: Frontiers in Immunology

    Article Title: Modulation of IRF7-driven transcription as a strategy to control HIV-1 latency

    doi: 10.3389/fimmu.2026.1735192

    Figure Lengend Snippet: Pacritinib blocks HIV latency reversal in an IRF7-dependent manner. (A) Left panel, WB of a representative experiment of HL60 cells treated with PMA or with a dose response of pacritinib (Pac) immunoblotted with anti-pJAK2, anti-pSTAT1, anti-IRF7 and anti-GAPDH antibodies. Middle panel, bar plots represent the mean quantification of IRF7 bands obtained by densitometry analysis of three independent experiments. Values were normalized to that of GAPDH used as a loading control and relativized to the untreated condition (ND). Right panel, relative mRNA expression of IRF7 gene expression measured by quantitative RT-PCR and normalized to GAPDH. (B, C) WB of a representative experiment in J-HIG (B) and HL-HIG (C) cells treated with PMA, pacritinib or fedratinib (Fed), alone or in combination, and immunoblotted with anti-IRF7 and anti-GAPDH antibodies. Bar plots represent the mean quantification of bands obtained by densitometry analysis of 3 independent experiments. (D, E) Correlation plots of IRF7 and gene expression versus HIV-1 latency reversal capacity of PMA or JAK2i-treated J-HIG and HL-HIG cells. (F) Transactivation assay as measured by luciferase expression in TZM-bl cells transfected to overexpress IRF7 protein and 24 hours after drug treatment. Luciferase values were normalized within each transfection condition (mock or IRF7 overexpression, left panel), and overexpression of IRF7 confirmed by WB ( right panel). PMA: phorbol 12-myristate 13-acetate (0,1µg/mL); Pac: pacritinib (1µM); Fed: fedratinib (5µM); Vor: vorinostat (5µM). Data expressed as means ± SD values from at least three independent experiments normalized to the no drug (ND) condition *p<0.05; **p<0.005; ***p<0.0001.

    Article Snippet: The human cell lines HL60, Jurkat, MOLT-4 and U937 were obtained from ATCC (Gaithersburg, MD).

    Techniques: Control, Expressing, Gene Expression, Quantitative RT-PCR, Transactivation Assay, Luciferase, Transfection, Over Expression